Journal: Molecular Pain

Article Title: Antidepressants inhibit P2X 4 receptor function: a possible involvement in neuropathic pain relief

doi: 10.1186/1744-8069-5-20

Figure Lengend Snippet: Effect of antidepressants on the ATP-evoked [Ca 2+ ] i response via rat P2X 4 receptors . Three rounds of ATP stimulation (30 μM, 20 sec) induced [Ca 2+ ] i response in a reproducible fashion (a). Effect of pretreatment of cells with EGTA (500 μM, 10 min) (b) or with paroxetine (10 μM, 10 min) (c) on [Ca 2+ ] i response evoked by the second ATP stimulation. Traces indicate 340/380 fura-2 emission ratios averaged from (a) 48 cells, (b) 66 cells and (c) 18 cells obtained from each representative experiment. Effect of pretreatment of cells with TNP-ATP, antidepressants and anticonvulsants (10 μM, 10 min) on the ATP-evoked [Ca 2+ ] i response via rat P2X 4 receptors (d). Paroxetine dose-dependently inhibited the [Ca 2+ ] i response via rat P2X 4 receptors with an IC 50 value of 2.45 μM for 30 μM ATP stimulation (e). ATP dose-response curve was generated in the presence of increasing concentrations of paroxetine (f). Data are means ± SEM of at least 10 cells (d, e and f).

Article Snippet: Carbamazepine (Sigma-Aldrich) and paroxetine hydrochloride (Toronto Research Chemicals Inc.) were dissolved in 0.1% dimethyl sulfoxide (DMSO) in BSS.

Techniques: Generated

Journal: Molecular Pain

Article Title: Antidepressants inhibit P2X 4 receptor function: a possible involvement in neuropathic pain relief

doi: 10.1186/1744-8069-5-20

Figure Lengend Snippet: Effect of antidepressants on ATP-evoked [Ca 2+ ] i response via human P2X 4 receptors . Effect of pretreatment of cells with antidepressants (10 μM, 10 min) on the ATP-evoked [Ca 2+ ] i response via human P2X 4 receptors (a). Paroxetine dose-dependently inhibited the ATP-evoked [Ca 2+ ] i response via human P2X 4 receptors with an IC 50 value of 1.87 μM for 30 μM ATP stimulation (b). ATP dose-response curve was generated in the presence of increasing concentrations of paroxetine (c). Data are means ± SEM of at least 3 cells (a, b and c).

Article Snippet: Carbamazepine (Sigma-Aldrich) and paroxetine hydrochloride (Toronto Research Chemicals Inc.) were dissolved in 0.1% dimethyl sulfoxide (DMSO) in BSS.

Techniques: Generated

Journal: Molecular Pain

Article Title: Antidepressants inhibit P2X 4 receptor function: a possible involvement in neuropathic pain relief

doi: 10.1186/1744-8069-5-20

Figure Lengend Snippet: Effect of antidepressants on ATP-evoked currents in 1321N1 cells expressing rat P2X 4 receptors and primary microglia . Paroxetine (10 μM, 10 min) inhibited ATP (30 μM, 10 sec) evoked currents in rat P2X 4 receptor-expressed 1321N1 cells (a and b) and primary micloglia (c and d) (*** p < 0.001; paroxetine vs. vehicle, unpaired t-test). Data are means ± SEM of more than three separate experiments.

Article Snippet: Carbamazepine (Sigma-Aldrich) and paroxetine hydrochloride (Toronto Research Chemicals Inc.) were dissolved in 0.1% dimethyl sulfoxide (DMSO) in BSS.

Techniques: Expressing

Journal: Molecular Pain

Article Title: Antidepressants inhibit P2X 4 receptor function: a possible involvement in neuropathic pain relief

doi: 10.1186/1744-8069-5-20

Figure Lengend Snippet: Effect of intrathecal administration of paroxetine on the decrease in the PWT after nerve injury . A significant antiallodynic effect was observed following intrathecal administration of paroxetine at doses of 3 nmol and 10 nmol at day 7 (a), and 3 nmol at day 14 (b) after nerve injury (** p < 0.01 and * p < 0.05; paroxetine 3 nmol vs. control, # p < 0.05; paroxetine 10 nmol vs. control by a Dunn's multiple comparison test after a Kruskal-Wallis test). Data are means ± SEM of 5–8 rats.

Article Snippet: Carbamazepine (Sigma-Aldrich) and paroxetine hydrochloride (Toronto Research Chemicals Inc.) were dissolved in 0.1% dimethyl sulfoxide (DMSO) in BSS.

Techniques: Control, Comparison

Journal: Molecular Pain

Article Title: Antidepressants inhibit P2X 4 receptor function: a possible involvement in neuropathic pain relief

doi: 10.1186/1744-8069-5-20

Figure Lengend Snippet: Effect of intrathecal co-administration of 5-HT receptor blockers with paroxetine on the decrease in the PWT after nerve injury . No significant change was observed by intrathecal co-administration of 100 nmol WAY100635, 30 nmol ketanserin or 30 nmol ondansetron with 3 nmol paroxetine compared with 3 nmol paroxetine alone. ( p > 0.05 by a Dunn's multiple comparison test after a Friedman test.) Data are means ± SEM of 5–6 rats.

Article Snippet: Carbamazepine (Sigma-Aldrich) and paroxetine hydrochloride (Toronto Research Chemicals Inc.) were dissolved in 0.1% dimethyl sulfoxide (DMSO) in BSS.

Techniques: Comparison

Journal: Molecular Pain

Article Title: Antidepressants inhibit P2X 4 receptor function: a possible involvement in neuropathic pain relief

doi: 10.1186/1744-8069-5-20

Figure Lengend Snippet: Effect of spinal 5-HT depletion on the antiallodynic effect of paroxetine . 5-HT immunoreactivity in the dorsal horn of the spinal cord nine days after intrathecal injection of either saline or 5,7-dihydroxytryptamine (a). A marked reduction in the number of 5-HT immunoreactive fibres was observed in the dorsal horn of the L5 spinal cord after 5,7-DHT treatment compared with the saline-treated group. No significant change in the antiallodynic effect of paroxetine was observed in the 5,7-DHT-treated group compared with the saline-treated group (b). ( p > 0.05 by a Dunn's multiple comparison test after a Friedman test.) Data are means ± SEM of 5–6 rats.

Article Snippet: Carbamazepine (Sigma-Aldrich) and paroxetine hydrochloride (Toronto Research Chemicals Inc.) were dissolved in 0.1% dimethyl sulfoxide (DMSO) in BSS.

Techniques: Injection, Saline, Comparison

Journal: The Journal of Biological Chemistry

Article Title: Ceramide-CD300f Binding Inhibits Lipopolysaccharide-induced Skin Inflammation *

doi: 10.1074/jbc.M116.768366

Figure Lengend Snippet: Ceramide-CD300f binding inhibited the release of chemical mediators from LPS-stimulated mast cells and neutrophils in vitro . A and B , the levels of MIP2 or LTC4 released from WT or CD300f −/− BMMC ( A ) or neutrophils ( B ) stimulated with 100 ng/ml LPS for 6 h. Cells were preincubated on plates coated with ceramide, PC, or vehicle for 1 h before stimulation. Data are representative of three independent experiments. Means ± S.D. are plotted. *, p < 0.01 (Student's t test).

Article Snippet: Cytokines were obtained from R&D Systems, and C-24 ceramide was obtained from Toronto Research Chemicals, Inc. 1,2-Dipalmitoyl- sn -glycero-3-phosphocholine (PC) was obtained from Echelon Biosciences Inc.

Techniques: Binding Assay, In Vitro

Journal: The Journal of Biological Chemistry

Article Title: Ceramide-CD300f Binding Inhibits Lipopolysaccharide-induced Skin Inflammation *

doi: 10.1074/jbc.M116.768366

Figure Lengend Snippet: Ceramide-CD300f binding inhibits LPS-induced skin inflammation. A and B , dye extravasation in ears of WT and CD300f −/− mice 1 h after intradermal injection with LPS. OD , optical density. C and D , the number of neutrophils recruited to skin pouches of WT and CD300f −/− mice 4 h after intradermal injection with LPS. A–D , ,ice intradermally injected with CD300f-Fc or control Fc ( A ), ceramide antibody or control antibody ( B and C ), or vesicles containing ceramide, PC, or vehicle ( D ) 1 h before LPS injection. Data are representative of two independent experiments. Means ± S.D. are plotted. *, p < 0.01 (Student's t test).

Article Snippet: Cytokines were obtained from R&D Systems, and C-24 ceramide was obtained from Toronto Research Chemicals, Inc. 1,2-Dipalmitoyl- sn -glycero-3-phosphocholine (PC) was obtained from Echelon Biosciences Inc.

Techniques: Binding Assay, Injection, Control